English speakers usually talk about travelling "by" car, "by" train, or sometimes "on" a bus or a plane. This means you have to choose between a few different prepositions depending on the vehicle.
Italian makes this much easier. When you want to express a generic mode of transportation, you generally just use the preposition in. Whether you are flying, driving, or taking public transit, the structure remains exactly the same.
Word list
aereomA1airplanein aereo = by planeautofA1carin auto = by carautobusmA1busin autobus = by busbicifA1bicyclein bici = by bikebiciclettafA1bicyclein bicicletta = by bicyclebusmA1busin bus = by busmacchinafA1carin macchina = by cartrenomA1trainin treno = by trainaeroplanomA2airplanein aeroplano = by planeambulanzafA2ambulancein ambulanza = by ambulanceautomobilefA2carin automobile = by carbarcafA2boatin barca = by boatcamionmA2truckin camion = by truckmetrofA2subwayin metro = by subwaymetropolitanafA2subwayin metropolitana = by subwaymotofA2motorcyclein moto = by motorcyclemotociclettafA2motorcyclein motocicletta = by motorcyclenavefA2shipin nave = by shippullmanmA2busin pullman = by bustaximA2taxiin taxi = by taxitrammA2tramin tram = by tramastronavefB1spaceshipin astronave = by spaceshipfurgonemB1vanin furgone = by vanpick-upmB1pickup truckin pick-up = by pickup truckscialuppa di salvataggiofB1life boatin scialuppa di salvataggio = by lifeboatsottomarinomB1submarinein sottomarino = by submarinetraghettomB1ferryin traghetto = by ferryelicotteromB2helicopterin elicottero = by helicopterkayakmB2kayakin kayak = by kayaklimousinefB2limousinein limousine = by limousinenave cargofB2cargo shipin nave cargo = by cargo shipnave da caricofB2cargo shipin nave da carico = by cargo shippullmanmB2coachin pullman = by coachyachtmB2yachtin yacht = by yachtcanoafC1canoein canoa = by canoecarrozzafC1carriagein carrozza = by carriagecorrierafC1coachin corriera = by coachnave a vaporefC1steamshipin nave a vapore = by steamshippanfilomC1yachtin panfilo = by yachtpiroscafomC1steamshipin piroscafo = by steamshipslittafC1sledin slitta = by sleigh
How it works
To say you are travelling by a certain vehicle, simply place in right before the noun. You do not need to add a definite article like il or la.
This rule covers everyday transport like a macchina (car), auto (car), treno (train), autobus (bus), and aereo (airplane).
Vado a Roma in treno.
(I am going to Rome by train.)
Preferisci viaggiare in aereo o in macchina?
(Do you prefer travelling by plane or by car?)
Loro vanno a scuola in autobus.
(They go to school by bus.)
Two wheels, water, and city transit
This handy rule extends to almost everything you can ride. You use it for city transit like the tram (tram), taxi (taxi), and metro (subway). It also applies to two-wheeled transport like a bicicletta (bicycle) or moto (motorcycle), as well as water vessels like a barca (boat), nave (ship), or traghetto (ferry).
Oggi vado al lavoro in bicicletta.
(Today I am going to work by bicycle.)
Torniamo a casa in taxi.
(Let's go back home by taxi.)
Andiamo in Sardegna in traghetto.
(We are going to Sardinia by ferry.)
Watch out
There are two important exceptions to keep in mind. First, if you are not using a vehicle at all, the preposition changes. To say you are going on foot, you must use a piedi. Similarly, travelling on horseback is a cavallo.
Non ho la macchina, vado a piedi.
(I do not have the car, I am going on foot.)
Second, the in rule only applies when you are talking about a generic method of travel. If you need to specify exactly which vehicle you are taking, you must use the preposition con (with) combined with a definite article.
Vado a Milano con il treno delle otto.
(I am going to Milan on the eight o'clock train.)
Summary
To talk about how you get from point A to point B, use the preposition in right before the vehicle without an article. Just remember to switch to a for walking or riding a horse, and use con with a definite article if you need to point out exactly which specific vehicle you are taking.